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enzyme linked immunospot elispot assay  (Cellular Technology Ltd)


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    Structured Review

    Cellular Technology Ltd enzyme linked immunospot elispot assay
    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed <t>by</t> <t>enzyme-linked</t> immunospot <t>(ELISpot)</t> assay. Data were shown as mean ± SD (n = 3).
    Enzyme Linked Immunospot Elispot Assay, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 929 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enzyme+linked+immunospot+elispot+assay/Human+IFN-%CE%B3+Single-Color+ELISPOT/pmc12926576-337-88-89
    Average 96 stars, based on 929 article reviews
    enzyme linked immunospot elispot assay - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy"

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.018

    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).
    Figure Legend Snippet: In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Techniques Used: In Vivo, Activation Assay, Injection, Flow Cytometry, Enzyme-linked Immunospot

    Related Articles

    Enzyme-linked Immunospot:

    Article Title: Perineural delivery of AAV2/9 in non-human primates is a safe and efficient route for gene therapy in Charcot-Marie-Tooth diseases
    Article Snippet: .. It is based on an enzyme-linked immunospot (ELISpot) assay and performed according to the manufacturer’s recommendations (MABTECH). ..

    Article Title: Durable hematopoiesis and tolerance after vertebral bone marrow transplant from a deceased lung transplant donor
    Article Snippet: .. Enzyme-linked immunospot (ELISPOT) assay was performed according to the manufacturer’s brochure (MilliporeSigma). .. TCRβ repertoire “immunosequencing” and bulk RNA sequencing were performed by Adaptive Biotechnologies or at the Health Sciences Sequencing Core at UPMC Children’s Hospital of Pittsburgh, respectively.

    Article Title: Subcutaneous white adipose tissue–derived extracellular vesicles maintain intestinal homeostasis via IgA biosynthesis in aging mice
    Article Snippet: Bacterial pellets were fixed in 2% 6 paraformaldehyde for 15 min, and then blocked with 5% normal rat serum for 10 min. Bacteria were stained with SYTO® BC green fluorescent dye (5 mM solution in DMSO, ThermoFisher Scientific, Cat# S34855) for 20 min. After washing twice with PBS, bacteria were stained with an anti-IgA antibody on ice for 30 min. Quantification of immunoglobulin-secreting cells (Ig-SC). .. SFB lysates were prepared from the feces of SFB-monoassociated mice by centrifugation through a Nycodenz gradient (Sigma, Cat#D2158) and Ig-SC from LPL were isolated (described above) and assessed in the enzyme-linked immunospot (ELISpot) assay using a multiscreen HTS 96-well plates (Millipore, Cat# MSHVN4B10) as described (4). .. Briefly, multiscreen HTS 96-well plates were coated with 100 μl/well of goat anti-mouse Ig (H+L) (Invitrogen; Cat#88-50450-22) or bacterial sonicate (40 μg/ml) overnight at 4°C to determine total and bacterium-specific Ig-SCs, respectively.

    Article Title: The modified mRNA vaccine protects immunocompromised AG129 mice from lethal challenge and multi-tissue infection by Zika virus.
    Article Snippet: .. Enzyme-linked immunospot (ELISpot) assay To evaluate cell-mediated immunity, we used Mouse IL-2 and IFN-γ kit (MABTECH, 3441-4APW-10 and 3321-4APW-10). ..

    Article Title: Nanoadjuvant-Mediated EV-Derived Artificial APC to Trigger Protective Immunity Against Virus Infection.
    Article Snippet: Antigen presentation is a crucial process for vaccine-induced immunity, as it determines the efficiency of a vaccine in eliciting effective antigen-specific immune responses by simultaneously processing antigens and presenting them to T cells.. A nano-APC is designed and proposed to bypass the antigen processing step by antigen-presenting cells (APCs) in vivo, directly eliciting effective antigen-specific cellular immunity to protect against lethal viral infections.. Extracellular vesicles (EVs) equipped with essential moieties for antigen presentation are prepared by incubating antigen-loaded self-adjuvanting chitosan nanoparticles (nChi) with naïve APCs. nChi enhances antigen uptake, activates stimulatory factors, and promotes EV production.

    Article Title: Ectopic CD11c Drives SMAD3-Mediated Aberrant Antigen Presentation and Epithelial–Mesenchymal Transition in Esophageal Squamous Cell Carcinoma
    Article Snippet: .. CD4 + T cells were cultured overnight in conditional medium containing 10% FBS and IL-2 in a 24-chambered plate and then cocultured with or without ESCC cells for 24 h. CD4 + T cells were then collected immediately for enzyme-linked immunospot (ELISpot) assay, with the Human IL-10 ELISpot Set (BD Biosciences, Cat. #551018), to enumerate the number of IL-10-producing T cells. ..

    Article Title: A lipid nanoparticle-based mRNA vaccine elicits immunity against porcine circovirus type 2 in mice.
    Article Snippet: Data for all samples were acquired on a BD FACSymphony flow cytometer (BD Biosciences) and analyzed using FlowJo software (v10.8). .. Antigen-specific T cell responses were quantitatively evaluated using an enzyme-linked immunospot (ELISpot) assay for IFN-γ (Mabtech, Cat: CVL-KIT30236) and interleukin 4 (IL-4; Mabtech, Cat. CVL-KIT30231) secretion, performed with a commercial ELISpot kit in strict accordance with the manufacturer’s standardized protocol (45). ..

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy
    Article Snippet: .. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. ..

    Centrifugation:

    Article Title: Subcutaneous white adipose tissue–derived extracellular vesicles maintain intestinal homeostasis via IgA biosynthesis in aging mice
    Article Snippet: Bacterial pellets were fixed in 2% 6 paraformaldehyde for 15 min, and then blocked with 5% normal rat serum for 10 min. Bacteria were stained with SYTO® BC green fluorescent dye (5 mM solution in DMSO, ThermoFisher Scientific, Cat# S34855) for 20 min. After washing twice with PBS, bacteria were stained with an anti-IgA antibody on ice for 30 min. Quantification of immunoglobulin-secreting cells (Ig-SC). .. SFB lysates were prepared from the feces of SFB-monoassociated mice by centrifugation through a Nycodenz gradient (Sigma, Cat#D2158) and Ig-SC from LPL were isolated (described above) and assessed in the enzyme-linked immunospot (ELISpot) assay using a multiscreen HTS 96-well plates (Millipore, Cat# MSHVN4B10) as described (4). .. Briefly, multiscreen HTS 96-well plates were coated with 100 μl/well of goat anti-mouse Ig (H+L) (Invitrogen; Cat#88-50450-22) or bacterial sonicate (40 μg/ml) overnight at 4°C to determine total and bacterium-specific Ig-SCs, respectively.

    Isolation:

    Article Title: Subcutaneous white adipose tissue–derived extracellular vesicles maintain intestinal homeostasis via IgA biosynthesis in aging mice
    Article Snippet: Bacterial pellets were fixed in 2% 6 paraformaldehyde for 15 min, and then blocked with 5% normal rat serum for 10 min. Bacteria were stained with SYTO® BC green fluorescent dye (5 mM solution in DMSO, ThermoFisher Scientific, Cat# S34855) for 20 min. After washing twice with PBS, bacteria were stained with an anti-IgA antibody on ice for 30 min. Quantification of immunoglobulin-secreting cells (Ig-SC). .. SFB lysates were prepared from the feces of SFB-monoassociated mice by centrifugation through a Nycodenz gradient (Sigma, Cat#D2158) and Ig-SC from LPL were isolated (described above) and assessed in the enzyme-linked immunospot (ELISpot) assay using a multiscreen HTS 96-well plates (Millipore, Cat# MSHVN4B10) as described (4). .. Briefly, multiscreen HTS 96-well plates were coated with 100 μl/well of goat anti-mouse Ig (H+L) (Invitrogen; Cat#88-50450-22) or bacterial sonicate (40 μg/ml) overnight at 4°C to determine total and bacterium-specific Ig-SCs, respectively.

    In Vitro:

    Article Title: Nanoadjuvant-Mediated EV-Derived Artificial APC to Trigger Protective Immunity Against Virus Infection.
    Article Snippet: Antigen presentation is a crucial process for vaccine-induced immunity, as it determines the efficiency of a vaccine in eliciting effective antigen-specific immune responses by simultaneously processing antigens and presenting them to T cells.. A nano-APC is designed and proposed to bypass the antigen processing step by antigen-presenting cells (APCs) in vivo, directly eliciting effective antigen-specific cellular immunity to protect against lethal viral infections.. Extracellular vesicles (EVs) equipped with essential moieties for antigen presentation are prepared by incubating antigen-loaded self-adjuvanting chitosan nanoparticles (nChi) with naïve APCs. nChi enhances antigen uptake, activates stimulatory factors, and promotes EV production.

    Cell Culture:

    Article Title: Ectopic CD11c Drives SMAD3-Mediated Aberrant Antigen Presentation and Epithelial–Mesenchymal Transition in Esophageal Squamous Cell Carcinoma
    Article Snippet: .. CD4 + T cells were cultured overnight in conditional medium containing 10% FBS and IL-2 in a 24-chambered plate and then cocultured with or without ESCC cells for 24 h. CD4 + T cells were then collected immediately for enzyme-linked immunospot (ELISpot) assay, with the Human IL-10 ELISpot Set (BD Biosciences, Cat. #551018), to enumerate the number of IL-10-producing T cells. ..

    Flow Cytometry:

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy
    Article Snippet: .. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. ..



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    Image Search Results


    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Journal: Bioactive Materials

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    doi: 10.1016/j.bioactmat.2026.02.018

    Figure Lengend Snippet: In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Article Snippet: Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay.

    Techniques: In Vivo, Activation Assay, Injection, Flow Cytometry, Enzyme-linked Immunospot